Mark Holodniy

Publication Details

  • Comparative effectiveness of dried plasma HIV-1 viral load testing in Brazil using ViveST for sample collection JOURNAL OF CLINICAL VIROLOGY Zanoni, M., Cortes, R., Diaz, R. S., Sucupira, M. C., Ferreira, D., Inocencio, L. A., Vilhena, C., Loveday, C., Lloyd, R. M., Holodniy, M. 2010; 49 (4): 245-248

    Abstract:

    Utilization of dried plasma for HIV-1 viral load testing would significantly decrease sample shipping costs.To describe the precision and reproducibility of ViveST(®) (ST) as a transportation method for shipping specimens for HIV-1 viral load (VL) testing.Thirty clinical plasma samples were used to generate replicate samples with HIV VL values of 4 log(10), 3 log(10) and 2 log(10) copies/mL for reproducibility testing and an additional 299 samples with HIV VL <50 copies/mL (99); 1.7 log(10) to 3.99 log(10) (100); and 4 log(10) to 5.99 log(10)/mL (100) were used to compare ViveST to frozen plasma samples using the VERSANT(®) HIV-1 RNA 3.0 Assay. Results were compared using Student t-test, Pearson correlation and Bland-Altman analyses.Mean intra-assay variance among frozen and dried plasma triplicates was 0.15 log(10) and 0.09 log(10) copies/mL respectively (n=10, P=NS). Compared to frozen plasma, there was a mean reduction of 0.3 log(10), 0.27 log(10), and 0.35 log(10) copies/mL at the 4 log(10), 3 log(10), and 2 log(10) copy/mL samples respectively (n=30, all comparisons, P<0.01). Overall correlation between 299 frozen and ViveST samples was r=0.97, where 12 of 99 undetectable frozen VL were positive with ST, and 12 of 200 frozen detectable VL were undetectable with ViveST (mean VL 2.1, 1.9 log(10) copies/mL respectively).HIV-1 viral load results using ViveST were reproducible, correlated well with frozen plasma, though yielding minimally lower values. Our data suggest that dried plasma for HIV-1 VL testing using ViveST has promise for use in HIV clinical practice.

    View details for DOI 10.1016/j.jcv.2010.08.017

    View details for Web of Science ID 000284105300004

    View details for PubMedID 20880740

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