C. Garrison Fathman

Publication Details

  • T cell receptor (TCR)-mediated repertoire selection and loss of TCR V beta diversity during the initiation of a CD4(+) T cell response in vivo JOURNAL OF EXPERIMENTAL MEDICINE Fasso, M., Anandasabapathy, N., Crawford, F., Kappler, J., Fathman, C. G., Ridgway, W. M. 2000; 192 (12): 1719-1730


    We recently described a novel way to isolate populations of antigen-reactive CD4(+) T cells with a wide range of reactivity to a specific antigen, using immunization with a fixed dose of nominal antigen and FACS((R)) sorting by CD4(high) expression. Phenotypic, FACS((R)), functional, antibody inhibition, and major histocompatibility complex-peptide tetramer analyses, as well as T cell receptor Vbeta sequence analyses, of the antigen-specific CD4(high) T cell populations demonstrated that a diverse sperm whale myoglobin 110-121-reactive CD4(+) T cell repertoire was activated at the beginning (day 3 after immunization) of the immune response. Within 6 d of immunization, lower affinity clones were lost from the responding population, leaving an expanded population of oligoclonal, intermediate affinity (and residual high affinity) T cells. This T cell subset persisted for at least 4 wk after immunization and dominated the secondary immune response. These data provide evidence that CD4(+) T cell repertoire selection occurs early in the immune response in vivo and suggest that persistence and expansion of a population of oligoclonal, intermediate affinity T cells is involved in CD4(+) T cell memory.

    View details for Web of Science ID 000166013100005

    View details for PubMedID 11120769

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