Mark Holodniy

Publication Details

  • DETECTION AND QUANTIFICATION OF HUMAN-IMMUNODEFICIENCY-VIRUS RNA IN PATIENT SERUM BY USE OF THE POLYMERASE CHAIN-REACTION JOURNAL OF INFECTIOUS DISEASES Holodniy, M., Katzenstein, D. A., Sengupta, S., Wang, A. M., CASIPIT, C., Schwartz, D. H., Konrad, M., Groves, E., Merigan, T. C. 1991; 163 (4): 862-866

    Abstract:

    Human immunodeficiency virus (HIV) RNA was detected and quantified in the serum of HIV-seropositive individuals using the polymerase chain reaction (PCR) and a nonisotopic enzyme-linked affinity assay. Of 55 HIV-infected patients who were not receiving therapy, serum HIV RNA was detected in 9 of 19 who were asymptomatic, 11 of 16 with AIDS-related complex (ARC), and 18 of 20 with AIDS, with copy numbers ranging from 10(2) to greater than or equal to 5 x 10(4) 200 microliters of serum based on a relationship between absorbance and known copy number of gag gene RNA. Linear regression analysis demonstrated a correlation between infectious titer in 42 patient sera cocultured with donor peripheral blood mononuclear cells (PBMC) and PCR product absorbance (r = .70, P less than .01). Serum HIV RNA detected by PCR also correlated with serum p24 antigen positivity, CD4 counts less than 400/mm3, and the presence of HIV-related symptoms or disease. Quantification of infectious HIV RNA in cell-free serum by PCR may be useful as a marker for for disease progression or in monitoring antiviral therapy.

    View details for Web of Science ID A1991FD93900031

    View details for PubMedID 2010639

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