Bryan Sun

Publication Details

  • Intersection of the RNA interference and X-inactivation pathways SCIENCE Ogawa, Y., Sun, B. K., Lee, J. T. 2008; 320 (5881): 1336-1341

    Abstract:

    In mammals, dosage compensation is achieved by X-chromosome inactivation (XCI) in the female. The noncoding Xist gene initiates silencing of the X chromosome, whereas its antisense partner Tsix blocks silencing. The complementarity of Xist and Tsix RNAs has long suggested a role for RNA interference (RNAi). Here, we report that murine Xist and Tsix form duplexes in vivo. During XCI, the duplexes are processed to small RNAs (sRNAs), most likely on the active X (Xa) in a Dicer-dependent manner. Deleting Dicer compromises sRNA production and derepresses Xist. Furthermore, without Dicer, Xist RNA cannot accumulate and histone 3 lysine 27 trimethylation is blocked on the inactive X (Xi). The defects are partially rescued by truncating Tsix. Thus, XCI and RNAi intersect, down-regulating Xist on Xa and spreading silencing on Xi.

    View details for DOI 10.1126/science.1157676

    View details for Web of Science ID 000256441100044

    View details for PubMedID 18535243

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