Gary Schoolnik

Publication Details

  • AN INDUCIBLE BUNDLE-FORMING PILUS OF ENTEROPATHOGENIC ESCHERICHIA-COLI SCIENCE Giron, J. A., HO, A. S., SCHOOLNIK, G. K. 1991; 254 (5032): 710-713

    Abstract:

    Enteropathogenic Escherichia coli (EPEC), a cause of childhood diarrhea, grow on the surface of the small intestine and on cultured epithelial cells as colonies of adherent bacteria. When propagated on solid medium containing blood or attached to HEp-2 cells, EPEC express ropelike bundles of filaments, termed bundle-forming pili (BFP), that create a network of fibers that bind together the individual organisms. BFP were found to be expressed by five EPEC serogroups, each harboring a approximately 92-kilobase plasmid previously known to be important for virulence in humans. When two of these strains were cured of this plasmid, they neither expressed BFP nor grew as adherent colonies. An antiserum to BFP reduced the capacity of EPEC to infect cultured epithelial cells. BFP are composed of a repeating subunit of 19,500 daltons, the amino-terminal amino acid sequence of this subunit is homologous to that of the toxin-coregulated pilin of Vibrio cholerae.

    View details for Web of Science ID A1991GN47400041

    View details for PubMedID 1683004

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