David A. Relman

Publication Details

  • VCAM-1 expression on CD8(+) cells correlates with enhanced anti-HIV suppressing activity JOURNAL OF IMMUNOLOGY DIAZ, L. S., Foster, H., Stone, M. R., Fujimura, S., Relman, D. A., Levy, J. A. 2005; 174 (3): 1574-1579

    Abstract:

    CD8(+) cells from HIV-infected individuals showing the CD8(+) cell noncytotoxic antiviral response unexpectedly revealed mRNA for VCAM-1, a cell surface molecule found on endothelial cells. Uninfected subjects had undetectable levels of VCAM-1 mRNA in their CD8(+) cells. Flow cytometry analysis showed that up to 12% of the CD8(+) cells from HIV-positive individuals expressed VCAM-1 compared with 0.8% of the CD8(+) cells of HIV-negative individuals. Enrichment of the CD8(+)VCAM-1(+) cell population and subsequent coculture with CD4(+) cells acutely infected with HIV-1 showed that the VCAM-1(+)CD8(+) cells were able to suppress viral replication with 50% less input cells than the unseparated CD8(+) cell population. This study demonstrates, for the first time, the expression of VCAM-1 on CD8(+) cells. Moreover, the CD8(+)VCAM-1(+) cells show enhanced CD8(+) cell noncytotoxic antiviral response activity that could have clinical importance in HIV infection.

    View details for Web of Science ID 000226571300054

    View details for PubMedID 15661918

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