Mark Holodniy

Publication Details

  • DETECTION AND QUANTIFICATION OF GENE AMPLIFICATION PRODUCTS BY A NONISOTOPIC AUTOMATED-SYSTEM BIOTECHNIQUES Holodniy, M., Winters, M. A., Merigan, T. C. 1992; 12 (1): 36-?

    Abstract:

    We describe in this report the ability to determine human immunodeficiency virus proviral copy number by an automated nonisotopic method. Our system utilizes a FACStarPLUS cell sorter, the GeneAmp PCR System 9600 and a Biomek 1000 robotic workstation. Linking these three machines allows cell populations to be sorted and the DNA amplified and quantitated with minimal technical effort. We have developed this system to quantitate proviral DNA copy number in sorted subpopulations of peripheral blood cells in one day.

    View details for Web of Science ID A1992GZ18400006

    View details for PubMedID 1531177

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