Lawrence Steinman

Publication Details

  • ANALYSIS OF THE T-CELL REPERTOIRE USING THE PCR AND SPECIFIC OLIGONUCLEOTIDE PRIMERS BIOTECHNIQUES Panzara, M. A., Gussoni, E., Steinman, L., Oksenberg, J. R. 1992; 12 (5): 728-735

    Abstract:

    Applications of PCR have revolutionized the field of immunogenetics particularly in studies of human leukocyte antigen class II polymorphism and more recently in the analysis of T cell receptor usage. The diversity of the variable region of the T cell receptor, however, has made it difficult to amplify the complete repertoire of T cell receptor transcripts. We have chosen to address this problem through the design of oligonucleotide primers specific for each of the known V alpha- and V beta-region T cell receptor families in order to characterize the T cell receptor repertoire. Using nonradioactive probes labeled with horse radish peroxidase, the system presented here allows for the rapid elucidation of the T cell receptor repertoire expressed in cells or tissue samples, such as those derived from autoimmune lesions. The identification of the T cell receptor repertoire involved in a pathogenic process can have therapeutic implications given the success of reversing experimental autoimmune disorders by directing specific forms of immunotherapy against V region gene products.

    View details for Web of Science ID A1992HT69600024

    View details for PubMedID 1515141

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