Andrew R. Hoffman

Publication Details

  • Activating and silencing histone modifications form independent allelic switch regions in the imprinted Gnas gene HUMAN MOLECULAR GENETICS Li, T., Vu, T. H., Yang, Y. W., Hu, J. F., Hoffman, A. R. 2004; 13 (7): 741-750

    Abstract:

    Activation and suppression of gene transcription is tightly controlled by epigenetic modifications. The imprinted Gnas1 gene region contains closely juxtaposed maternally expressed (Nesp) and paternally expressed (Nespas, Gnasxl, Exon 1A) transcripts, providing a unique opportunity to study how epigenetic modifications change in nucleosomes from active to silenced promoters. Using 30 polymorphic sites across the Gnas1 gene region in (C57BL/6JxMus spretus) F(1) mice and chromatin immunoprecipitation (ChIP) assays we identified two allelic switch regions (ASRs) that mark boundaries of epigenetic information. We show that activating signals (histone acetylation and methylation of H3 Lys4) and silencing signals (histone methylation of H3 Lys9 and DNA methylation) segregate independently across the ASRs and suggest that these ASRs allow the transcriptional elongation to proceed through the silenced domain of nearby imprinted promoters. We discuss these findings in light of recent progress in the conceptualization of nucleosome remodeling during transcriptional elongation and in the development of histone code.

    View details for DOI 10.1093/hmg/ddh081

    View details for Web of Science ID 000220179900007

    View details for PubMedID 14962976

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