David A. Relman

Publication Details

  • IDENTIFICATION OF UNCULTURED MICROORGANISMS - EXPANDING THE SPECTRUM OF CHARACTERIZED MICROBIAL PATHOGENS INFECTIOUS AGENTS AND DISEASE-REVIEWS ISSUES AND COMMENTARY Relman, D. A., FALKOW, S. 1992; 1 (5): 245-253

    Abstract:

    The combination of enzymatic nucleic acid amplification techniques with 16S rRNA-based molecular phylogeny has brought about a new approach to the identification of microbial pathogens that can not be cultivated in the laboratory. The applications of this experimental approach to bacillary angiomatosis and to Whipple's disease have revealed the presence of two previously uncharacterized organisms. These results suggest the existence of a far greater microbial diversity among human pathogens than has been so far appreciated with culture-dependent methods. PCR-based studies of aquatic environmental microbial communities have already reached similar conclusions. As a result, new and provocative questions are raised concerning the association of amplified 16S rRNA sequences with diseased tissue. The answers must await the results of further investigations and the expansion of sequence data bases.

    View details for Web of Science ID A1992KD52000003

    View details for PubMedID 1285351

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