Robert W. Shafer

Publication Details

  • Comparison of QIAamp HCV kit spin columns, silica beads, and phenol-chloroform for recovering human immunodeficiency virus type 1 RNA from plasma JOURNAL OF CLINICAL MICROBIOLOGY Shafer, R. W., Levee, D. J., Winters, M. A., Richmond, K. L., Huang, D., Merigan, T. C. 1997; 35 (2): 520-522

    Abstract:

    Human immunodeficiency virus type 1 (HIV-1) pol mutations are responsible for HIV-1 resistance to current antiretroviral drugs. HIV-1 RNA extraction with QIAamp HCV kit spin columns (Qiagen, Chatsworth, Calif.) followed by reverse transcription-PCR successfully recovered a 1,008-bp pol fragment from the plasma of 31 of 34 HIV-1-infected patients that was suitable for sequencing and recombinant-virus studies. The minimum HIV-1 RNA concentration required for gene recovery was 30 to 40 copies/ml, which was similar to the minimal HIV-1 RNA concentration required when phenol-chloroform or silica beads are used for RNA extraction.

    View details for Web of Science ID A1997WD08200042

    View details for PubMedID 9003633

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