Timothy Angelotti MD, PhD

Publication Details

  • Tissue-specific expression of splice variants of the mouse voltage-gated calcium channel alpha 2/delta subunit FEBS LETTERS Angelotti, T., Hofmann, F. 1996; 397 (2-3): 331-337


    Five different splice variants of mouse alpha2/delta subunit isoforms (alpha2a-e), which arose from various combinations of three alternatively spliced regions, were cloned with a combination of cDNA library screening and RT-PCR. Expression patterns and relative abundance of the various isoforms in mouse tissues were determined with an RNAse protection assay. Skeletal muscle and brain expressed single isoforms, alpha2a and alpha2b, respectively; however, the cardiovascular system expressed all five isoforms. Heart expressed mainly isoforms alpha2c and alpha2d while, in contrast to other species, aorta expressed predominantly alpha2a, the 'skeletal muscle' isoform. Smooth muscle-containing tissues expressed alpha2d and alpha2e. Thus, alpha2/delta isoforms are restricted in their tissue expression, suggesting an important functional role for the differentially spliced variants.

    View details for Web of Science ID A1996VV17100043

    View details for PubMedID 8955374

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