Lawrence Steinman

Publication Details

  • DIVERSE T-CELL RECEPTOR V-BETA GENE USAGE IN THE CENTRAL-NERVOUS-SYSTEM IN EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS JOURNAL OF IMMUNOLOGY Bell, R. B., Lindsey, J. W., Sobel, R. A., Hodgkinson, S., Steinman, L. 1993; 150 (9): 4085-4092

    Abstract:

    The repertoire of TCR V beta genes transcribed and expressed within the central nervous system was determined in mice with experimental allergic encephalomyelitis. Disease was induced in (PL/J x SJL/)F1 mice by immunizing with myelin basic protein-acetylated peptide 1-11, and mice were sacrificed at intervals from day 3 postimmunization to 3 wk after recovery from disease. Transcription of V beta genes was determined by reverse transcriptase polymerase chain reaction on RNA extracted from spinal cord, and expression of the V beta gene products was detected by immunohistochemistry with mAb specific for various V beta proteins. Multiple V beta genes were found to be transcribed and expressed in the central nervous system starting 7 days after immunization, and continuing up to 3 wk after clinical recovery. Preferential utilization of a single TCR V beta gene was not detected in the central nervous system at any time in the course of disease.

    View details for Web of Science ID A1993KY45200043

    View details for PubMedID 8473751

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