Lucy Tompkins

Publication Details

  • TRANSFORMATION OF HELICOBACTER-PYLORI BY ELECTROPORATION BIOTECHNIQUES Segal, E. D., Tompkins, L. S. 1993; 14 (2): 225-226

    Abstract:

    A highly efficient method of introducing DNA into an important human pathogen is reported here. Electroporation-mediated transformation of a laboratory-passaged isolate of Helicobacter pylori was successfully used to establish genetic mutants at a transformation frequency of > 10(5)/micrograms DNA. This method should be widely applicable to all isolates of H. pylori and may eliminate the variability reported when natural transformation was used on fresh clinical isolates.

    View details for Web of Science ID A1993KL40900023

    View details for PubMedID 8431287

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