Samuel Strober

Publication Details

  • HOMOGENEOUS ANTIGEN RECEPTOR BETA-CHAIN GENES IN CLONED CD4- CD8- ALPHA-BETA T-SUPPRESSOR CELLS JOURNAL OF IMMUNOLOGY SCHMIDTWOLF, I. G., Liang, O., DEJBAKHSHJONES, S., Wang, H., Cheng, L. R., Holm, B., Bell, R., Strober, S. 1993; 151 (10): 5348-5353

    Abstract:

    The rearrangements of beta-chain genes of the T cell Ag receptor were examined in 12 CD4- CD8- alpha beta + T cell lines derived from the spleen or thymus of neonatal or adult BALB/c mice. Eleven of the lines were cloned and established from six independent cloning procedures from different mice. Five cloned lines used V beta 9, four cloned lines used V beta 15, and two cloned lines used V beta 7. Nucleotide sequencing of the beta-chain genes showed that clones that used a given V beta were identically rearranged even when they were derived from independent cloning procedures. In the case of V beta 7 and V beta 15 all nucleotides in the V-D-J joining region were in the germ line configuration without N region additions. Rearrangements of the V beta 7, V beta 9, and V beta 15 genes were functional. Each V beta 15 clone also had a homogeneous rearrangement of the V beta 13 gene, which was nonfunctional. The predicted amino acid sequence of the joining regions of the V beta 7, V beta 9, and V beta 15 rearrangements showed homology in four of seven amino acids in the peptide contact region.

    View details for Web of Science ID A1993MF99500024

    View details for PubMedID 8228230

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