Gary Schoolnik

Publication Details

  • INFECTION-RATES, DISEASE FREQUENCY, PILIN GENE REARRANGEMENT, AND PILIN EXPRESSION IN CALVES INOCULATED WITH MORAXELLA-BOVIS PILIN-SPECIFIC ISOGENIC VARIANTS AMERICAN JOURNAL OF VETERINARY RESEARCH Ruehl, W. W., Marrs, C. F., George, L., BANKS, S. J., SCHOOLNIK, G. K. 1993; 54 (2): 248-253

    Abstract:

    Pili have been implicated as virulence factors that result in increased infectivity of Moraxella bovis, the causative agent of infectious bovine keratoconjunctivitis (IBK). Healthy calves' eyes were inoculated with I- or Q-piliate or nonpiliate M bovis Epp63 to compare the pathogenicity of these isogenic variants. Pathogenicity was determined by the rate of persistent M bovis infection and the prevalence of clinical IBK. Inoculation with M bovis expressing the Q pili resulted in the highest frequency of infection and IBK, whereas I-piliate M bovis elicited a lower rate and nonpiliate M bovis did not result in infection. In vivo pilin gene rearrangement and pilin-type switching were evaluated by DNA hybridization and immunoblot. Gene rearrangement and type switching varied dependently, and were observed only in eyes inoculated with Q-piliate M bovis. This study suggests that Q pili are specific for colonization of bovine corneal epithelium, whereas I pili enable maintenance of an established infection.

    View details for Web of Science ID A1993KJ76400009

    View details for PubMedID 8094276

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