Frank M. Longo

Publication Details

  • ENDOGENOUS AND EXOGENOUS FACTORS SUPPORT NEURONAL SURVIVAL AND CHOLINE-ACETYLTRANSFERASE ACTIVITY IN EMBRYONIC SPINAL-CORD CULTURES BRAIN RESEARCH MANTHORPE, M., Luyten, W., LONGO, F. M., VARON, S. 1983; 267 (1): 57-66

    Abstract:

    Dissociated 4-day (stage 23) chick embryo lumbar cord cells were cultured at low or high cell densities for 1 or 5 days in the presence or absence of added spinal neuronotrophic factor (supplied as RN22 Schwannoma conditioned medium, RCM). In low density, 1-day cultures neuronal survival was dependent on added RCM whereas by 5 days no neurons survived, even in the presence of RCM. In high density 1-day cultures a substantial neuronal population could survive even without added RCM and a large proportion of this neuronal population would survive for 5 days. When conditioned media from high density lumbar cord cultures was supplied to low density unsupplemented cultures, a similar level of 5-day neuronal survival resulted. However, no neurons survived in RCM-supplemented 5-day high density cultures, indicating the presence in RCM of a material toxic for the neurons. Both the RCM and the high density lumbar culture-conditioned medium supported considerable choline acetyltransferase activity indicating the presence within these cultures of motoneurons.

    View details for Web of Science ID A1983QS81900005

    View details for PubMedID 6860950

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