Matt van de Rijn

Publication Details

  • RECOGNITION OF HLA-A2 BY CYTO-TOXIC LYMPHOCYTES-T AFTER DNA TRANSFER INTO HUMAN AND MURINE CELLS SCIENCE VANDERIJN, M., Bernabeu, C., ROYERPOKORA, B., Weiss, J., Seidman, J. G., Devries, J., Spits, H., TERHORST, C. 1984; 226 (4678): 1083-1085

    Abstract:

    A gene coding for the major histocompatibility antigen HLA-A2 was transferred into human HLA-A2 negative M1 cells and murine L cells. Following transfection, these cells expressed molecules at the cell surface that are biochemically indistinguishable from HLA-A2 antigens on the human cell line JY from which the HLA-A2 gene was isolated. The M1A2 cells were recognized and lysed by a cytolytic T-cell clone specific for HLA-A2. The transfected L cells which express HLA-A2 in association with human beta 2-microglobulin were not lysed by this T-cell clone. The specific cytolysis of M1A2 cells could be inhibited by monoclonal antibodies to HLA-A2, and monoclonal antibodies to T3, T8, and LFA-1 on cytotoxic T lymphocytes. These results suggest that killing by allospecific T cells requires HLA-A2 antigens as well as other species-specific structures on the target cell surface.

    View details for Web of Science ID A1984TT08100028

    View details for PubMedID 6333726

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