Uta Francke

Publication Details

  • SINGLE-COPY SEQUENCE HYBRIDIZES TO POLYMORPHIC AND HOMOLOGOUS LOCI ON HUMAN X-CHROMOSOME AND Y-CHROMOSOME PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES Page, D., DEMARTINVILLE, B., Barker, D., Wyman, A., White, R., FRANCKE, U., Botstein, D. 1982; 79 (17): 5352-5356

    Abstract:

    Use of a 4.5-kilobase-pair (kb) segment of single-copy DNA from a human genomic library as a hybridization probe of genomic human DNAs revealed allelic Taq I restriction fragments 10.6, 11.8, and 14.6 kb long. Among 12 unrelated individuals, all 6 males exhibited the 14.6-kb fragment in addition to one of the other fragments. Three of the females displayed 10.6- and 11.8-kb fragments, and the other three displayed only one fragment length; none had the 14.6-kb fragment. Hybridization of this probe to Taq I-digested DNAs from human-rodent hybrid cell lines (which have partial complements of human chromosomes) demonstrated segregation of the 14.6-kb fragment with the human Y chromosome and segregation of the 10.6- and 11.8-kb fragments with the human X chromosome. Furthermore, hybridization of this probe to Taq I-digested DNAs from 48 members of a single kindred revealed Y-linked inheritance of the 14.6-kb fragment and X-linked inheritance of the 10.6- and 11.8-kb fragments. These experiments demonstrate homology between single-copy sequences on the human X and Y chromosomes.

    View details for Web of Science ID A1982PE86500050

    View details for PubMedID 6291041

Stanford Medicine Resources:

Footer Links: