Clare J. Twist

Publication Details

  • STUDIES OF CULTURED HUMAN LYMPHOCYTES-T .2. INITIATION OF PAIRED T-CELL AND B-CELL LINES FROM HEALTHY DONORS PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE Pauly, J., Russell, C. W., PIRELA, D. L., Twist, C. J., REINERTSON, R., Callahan, J., Minowada, J. 1983; 172 (3): 283-290

    Abstract:

    We report the sustained cultivation of both B- and T-lymphoblastoid cell lines from randomly selected healthy donors, and the results of studies defining the frequency with which these cell lines can be established. B-cell lines were initiated using the Epstein-Barr virus. Of 52 attempts, 40 B-cell lines (77% success) were obtained from 24 different donors. T-cell lines were started and propagated in long-term (greater than 100 days) cultures using the T-cell growth factor interleukin-2 (IL-2). Of 55 attempts, 54 (98%) were successful in initiating IL-2-dependent T-cell lines, and these were derived from 28 healthy adults. Likewise, of 45 attempts, 32 (71%) were successful in producing paired lines in which both the B-cell line and T-cell line were cultivated from a single blood collection (N = 22 donors). Phenotypic profiles of these lines were defined using multiple marker assays, including rosette formation, surface immunoglobulins, cytochemistry, karyotype, as well as xenoantisera and monoclonal antibodies defining different membrane antigens. This work demonstrates the feasibility of propagating paired human B and T lymphoblastoid lines suitable for many comparative immunobiological studies.

    View details for Web of Science ID A1983QG04100002

    View details for PubMedID 6189130

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