Steven Foung

Publication Details

  • HUMAN MONOCLONAL-ANTIBODIES TO RH0(D) VOX SANGUINIS Foung, S. K., Blunt, J. A., Wu, P. S., AHEARN, P., WINN, L. C., Engleman, E. G., GRUMET, F. C. 1987; 53 (1): 44-47


    B lymphocytes from Rh negative donors with serum anti-D antibodies were isolated and fused with the mouse-human heteromyeloma, SBC-H20, to produce hybridomas secreting IgM or IgG1 human monoclonal antibodies to D antigen. The IgM antibody in hybridoma supernatant agglutinates all normal D positive cells at the immediate spin phase of reactivity. Using concentrated IgM hybridoma supernatant of approximately 50 micrograms/ml, Du cells were also agglutinated. The IgG1 antibody reacts by indirect hemagglutination with all D and Du cells. Against Rh mosaics, different reactivity was noted for each antibody. Furthermore, D positive cells precoated with the IgG1 antibody inhibit the IgM direct hemagglutination, suggesting that the antibodies identify closely associated epitopes. These human monoclonal antibodies will be useful diagnostic reagents and, ultimately, should be useful in the prevention of Rh hemolytic disease of the newborn.

    View details for Web of Science ID A1987J436100009

    View details for PubMedID 3116771

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