Darrell Wilson

Publication Details

  • DEMONSTRATION OF INSULIN-LIKE GROWTH-FACTOR (IGF-I AND IGF-II) RECEPTORS AND BINDING-PROTEIN IN HUMAN-BREAST CANCER CELL-LINES BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS DeLeon, D. D., Bakker, B., Wilson, D. M., Hintz, R. L., Rosenfeld, R. G. 1988; 152 (1): 398-405

    Abstract:

    The insulin like growth factors (IGFs), potent mitogens for a variety of normal and transformed cells, have been reported to be secreted by several human breast cancer cell lines (BC). We have investigated the binding characteristics of IGF-I and -II in four human BC: MCF-7, T-47D, MDA 231 and Hs578T. Binding studies in microsomal membrane preparations detected high specific binding for both IGF in all four BC studied. Cross-linking with 125I-IGF-I, followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reduced conditions, revealed the presence of an alpha subunit of apparent Mr = 130,000 in MCF-7, T-47D and MDA 213 cells. When 125I-IGF-II was cross-linked, a major band of apparent Mr = 260,000 was seen in all BC. This band was inhibited by IGF-II, but not by insulin. Cross-linking of 125I-IGF-I to conditioned media from BC demonstrated the presence of three binding proteins of apparent Mr = 45,000, 36,000 and 29,000 in all BC but T-47D, in which the 36,000 band was not seen. These data demonstrate that BC possess classical receptors for both IGF-I and -II and, furthermore, that BC produce specific binding proteins for these growth factors.

    View details for Web of Science ID A1988M934800057

    View details for PubMedID 2451917

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