William Clusin, MD

Publication Details

  • EFFECT OF PLATELET-RELEASE PRODUCTS ON CYTOSOLIC CALCIUM IN CARDIAC MYOCYTES BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Chien, W. W., Mohabir, R., Newman, D., Leung, L. L., Clusin, W. T. 1990; 170 (3): 1121-1127

    Abstract:

    The effect of platelet release products on cytosolic calcium [( Ca++]i) was examined by monitoring the fluorescence of chick embryonic heart cells loaded with the fluorescent calcium indicator indo-1 AM. Cell free filtrate of platelet release products was obtained from rabbit platelets activated with thrombin or collagen. This filtrate caused a rapid increase in both systolic and diastolic [Ca++]i in a dose-dependent manner. The effect was not blocked by pretreating the platelets with aspirin or a thromboxane synthetase inhibitor. It was not mimicked by a thromboxane analog, or by several substances known to be released from platelets including ADP, serotonin, or platelet activating factor. Apyrase or ATP-gamma S had no effect on the activity. The responsible product was heat-sensitive, trypsin-sensitive, and partitioned into the aqueous phase of a chloroform suspension. It has a low molecular weight (less than 3kD) and is sensitive to 2-mercaptoethanol. Protease inhibitor appears to prolong the activity. These results suggest that trypsin-sensitive peptide(s) released from activated platelets can increase [Ca++]i in cardiac cells.

    View details for Web of Science ID A1990DV44800022

    View details for PubMedID 2390080

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