Michael Fischbein

Publication Details

  • Potential Role of gamma delta T Cell-Derived IL-17 in Acute Cardiac Allograft Rejection ANNALS OF THORACIC SURGERY Kimura, N., Nakae, S., Itoh, S., Merk, D. R., Wang, X., Gong, Y., Okamura, H., Chang, P. A., Adachi, H., Robbins, R. C., Fischbein, M. P. 2012; 94 (2): 542-548

    Abstract:

    Although ?? T cells are known to participate in the development of acute cardiac allograft rejection, the role of ?? T cells remains poorly understood. We hypothesized that ?? T cells contribute to acute allograft rejection thru interleukin (IL)-17 production.Donor hearts from FVB mice (H-2q) were heterotopically transplanted into C57BL/6-wild type (WT) and ?? T cell-deficient (TCR?-/-) recipient mice (H-2b). Overall graft survival was monitored. Graft infiltrating cell profile, including ?? T cell subtype, cytokine expression, and myeloperoxidase activity were measured by flow cytometry, TaqMan (Applied Biosystems, Carlsbad, CA) polymerase chain reaction, and myeloperoxidase assay, respectively, on postoperative days 3 and 6.Graft survival was prolonged in TCR?-/- recipients compared with WT controls. Graft infiltrating cells, including CD45+, CD4+, CD8+, and Gr1+ cells were significantly decreased in TCR?-/- recipients compared with WT. Donor hearts transplanted into TCR?-/- recipients had reduced IL-17 and IL-6 messenger RNA expression. Corroborating the gene expression, intracellular cytokine staining showed decreased IL-17 producing cells in TCR?-/- recipients. Finally, V?1+ and V?4+ T cells did not produce IL-17, although both represent 20% to 30% total graft infiltrating ?? T cells.The ?? T cells promote acute cardiac allograft rejection, presumably by producing IL-17. The ?? T cell depletion may prove beneficial in prolonging allograft survival by suppressing IL-17 production.

    View details for DOI 10.1016/j.athoracsur.2012.03.049

    View details for Web of Science ID 000306700100040

    View details for PubMedID 22560321

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