Stanford Fertility and Reproductive Medicine Center  


Video showing Intra Cytoplasmic Sperm Injection (ICSI)button
This video shows the injection of a sperm, loaded in the injection pipette on the right, into an egg (oocyte) being anchored on a holding pipette on the left.
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Day 3, 8-cell Embryo biopsybutton
This video shows the removal of a cell (blastomere) from an 8-cell embryo. A laser is used to breach the egg shell (zona pellucida). The blastomere is removed using a biopsy pipette.
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Day 5/6, Blastocyst biopsybutton
This video shows the real time removal of several outer cells of a Blastocyst (trophectoderm). The trophectoderm cells have “hatched” out of the zona pellucida allowing for easy access for biopsy.
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Video showing Intra Cytoplasmic Sperm Injection (ICSI)

Not shown in the video is the immobilization of the sperm and loading into the injection pipette.

Once the pipette is inserted into the oocyte, the egg fluid (ooplasm), is aspirated into the injection pipette and the pipette then advanced to stretch the egg membrane (oolemma) in order to deposit the sperm inside the egg once it breaks. Breakage can be visualized by the sudden movement of ooplasm in the injection pipette. The oocyte is rinsed and placed into culture for approximately 18hrs when fertilization status is determined.

Day 3, 8-cell Embryo biopsy

The embryo is biopsied in a special medium that makes the blastomeres less sticky to each other. Once the blastomere is in the biopsy pipette, the nucleus becomes visible as a round crater in the cell. The cell would then be expelled from the pipette and send for genetic analysis. The embryo is then rinsed and placed back into culture until the genetic information is available.

Day 5/6, Blastocyst biopsy:

The laser is fired to weaken the trophectoderm cell junctions in order to tease the cells off the embryo. Several cells are generally available to send for analysis. The blastocyst is rinsed and then placed back in culture for a fresh embryo transfer the next day.

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